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Related post: genic viruses, there has been no satisfactory explanation for this discrepant behavior. To understand these phenomenon model systems offering possible explanations must be developed and studied. We are optimistic that the Ad2/SV40 transformed hamster-cell system we are studying will be useful 18-10 in beginning to provide a definitive explanation of the essential factors associated with the conversion of normal cells to tumor cells by oncogenic viruses. The recently described phenomenon of RNA processing introduces a new level of regulation of molecular events in eukaryotic cells. The influence this regulatory system has on animal virus replication are currently being defined. Our studies thus far suggest that the virus-host cell systems associated with the replication of the nondefective Ad2-SV40 hybrid, Ad2+ND,, will be useful in studying the effect of RNA processing on the ability of this recombinant to replicate Buy Septilin in cells from different species. Information from such studies could be important in assessing and controlling the attenuation of viruses for the development of live virus vaccines. Publications Lewis, A. M., Jr. and Martin, R. G.: The oncogenicity of simian virus 40 deletion mutants that induce altered 17k t-proteins. Proc. Nat. Acad, of Sci . , 7_6: 4299-4302, 1979. Patch, C. T. , Hauser, J., Lewis, A. M. , Jr., Buy Septilin Online and Levine, A. S.: A method for determining the extent and copy number of overlapping and non-overlapping segments of integrated viral genomes. J. of Virol., 3j_: 575-578, 1979. Cook, J. L. and Lewis, A. M., Jr.: Age-related and thymus-dependent rejection of adenovirus 2 - transformed cell tumors in the Syrian hamster. Cancer Res., 39: 3335-3340, 1979, Lewis, A. M. , Jr. and Cook, J. L.: The association of tumor induction by ul- tra-violet light inactivated adenovirus 2-SV40 recombinants with a specific segment of SV40 DNA. J. of Nat. Cancer Inst. 63: 695-705, 1979. Cook, J. L., Kirkpatrick, C. H., Rabson, Septilin Himalaya A. S. and Lewis, A. M., Jr.: Rejec- tion of adenovirus 2-transformecl cell tumors and immune responsiveness in Syrian hamsters. Cancer Res. 39: 4949-4955, 1979. Lewis, A. M. , Jr. and Cook, J. L.: Presence of allograft-rejection resistance in simian virus 40 - transformed hamster cells and its possible role in tumor development. Proc. Nat. Acad. Sci. 77; 2886-2889, 1980. Cook, J. L., Hibbs, J. B., Jr. and Lewis, A. M., Jr.; Resistance of simian virus 40 - transformed hamster cells to the cytolytic effect of activated macrophages - a possible factor in species-specific oncogenicity. Proc. Nat. Acad. Sci. In press. Lewis, A. M. , Jr., Cook, J. L. , Kirkpatrick, C. H., and Rabson, A. S. : The implications of the different tumor-inducing capacities of adenovirus 2 and SV40 transformed hamster cells. Proceedings of the Symposium on Hamster Immune Responsiveness. In press. Cook, J. L., Hibbs, J. B. and Lewis, A. M., Jr.: A relationship between SV40- transformed cell susceptibility to macrophage killing and tumor induction in rodents. Proceedings of the Symposium on Hamster Immune Responsiveness. In press. 18-11 TMITHSONIAN SCIENCE INFORMATION EXCHANGE PK3JECT NUMBER (Oo NOT use this spacej U.S. DEPARTMENT OF HEALTH, EDUCATION, AND Himalaya Septilin WELFARE PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl -AT -00018-14 OSD PERIOD COVERED October 1. 1979 to September 30. 1980 TITLE OF PROJECT (80 characters or less) Biological and biochemical characterization of Septilin Tablets human papovaviruses NAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER PROFESSIONAL PERSONNEL ENGAGED ON THE PROJECT Principal Investigator: Kenneth K. Takemoto, Tatsuo Miyamura, Visiting Fellov/, and Kunito Yoshiike, Visiting Scientist - NIAID, OSD. COOPERATING UNITS (if any) , , „ ^,„^ „ _ r^^^^A Peter Howley, Ming-Fan Law, Francoise Rentier-Delrue, LP, NCI; George C. Fareed, Havjley Linke, UCLA, Los Angeles; Mark Israel, NIAID. lab/branch Office of the Scientific Director SECTION Virology Unit INSTITUTE AND LOCATION Nat ional Institute of Allergy and Infectious Diseases, Bethesda, Md. TOTAL MANYEARS: PROFESSIONAL: OTHER: CHECK APPROPRIATE BOX(ES) G (a) HUMAN SUBJECTS D (al) MINORS □ (a2) INTERVIEWS D (b) HUMAN TISSUES n (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) The human papovavirus JCV v^hich heretofore has been grown only in human fetal glia cells was successfully adapted to growth in readily available human Embryonic kidney cells. During the process of adaptation, defective viruses were generated which became Septilin Syrup the predominant type of virions ^^ ^he adapted virus pool The adapted virus formed plaques in HEK cells However, cloned virus stocks derived after plaque-purification still yielded 2 or more species of ? 'd A "he vfra/oNA was then molecularly cloned and analyzed by res rc- tion endonuclease cleavage and by electron microscopic studies of heterodupiex
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