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Related post: genic viruses, there has been no satisfactory explanation for this discrepant
behavior. To understand these phenomenon model systems offering possible
explanations must be developed and studied. We are optimistic that the
Ad2/SV40 transformed hamster-cell system we are studying will be useful
in beginning to provide a definitive explanation of the essential factors
associated with the conversion of normal cells to tumor cells by oncogenic
The recently described phenomenon of RNA processing introduces a new level of
regulation of molecular events in eukaryotic cells. The influence this
regulatory system has on animal virus replication are currently being defined.
Our studies thus far suggest that the virus-host cell systems associated with
the replication of the nondefective Ad2-SV40 hybrid, Ad2+ND,, will be useful
in studying the effect of RNA processing on the ability of this recombinant
to replicate Buy Septilin in cells from different species. Information from such studies
could be important in assessing and controlling the attenuation of viruses
for the development of live virus vaccines.
Lewis, A. M., Jr. and Martin, R. G.: The oncogenicity of simian virus 40
deletion mutants that induce altered 17k t-proteins. Proc. Nat. Acad, of Sci . ,
7_6: 4299-4302, 1979.
Patch, C. T. , Hauser, J., Lewis, A. M. , Jr., Buy Septilin Online and Levine, A. S.: A method for
determining the extent and copy number of overlapping and non-overlapping
segments of integrated viral genomes. J. of Virol., 3j_: 575-578, 1979.
Cook, J. L. and Lewis, A. M., Jr.: Age-related and thymus-dependent rejection
of adenovirus 2 - transformed cell tumors in the Syrian hamster. Cancer Res.,
39: 3335-3340, 1979,
Lewis, A. M. , Jr. and Cook, J. L.: The association of tumor induction by ul-
tra-violet light inactivated adenovirus 2-SV40 recombinants with a specific
segment of SV40 DNA. J. of Nat. Cancer Inst. 63: 695-705, 1979.
Cook, J. L., Kirkpatrick, C. H., Rabson, Septilin Himalaya A. S. and Lewis, A. M., Jr.: Rejec-
tion of adenovirus 2-transformecl cell tumors and immune responsiveness in
Syrian hamsters. Cancer Res. 39: 4949-4955, 1979.
Lewis, A. M. , Jr. and Cook, J. L.: Presence of allograft-rejection resistance
in simian virus 40 - transformed hamster cells and its possible role in tumor
development. Proc. Nat. Acad. Sci. 77; 2886-2889, 1980.
Cook, J. L., Hibbs, J. B., Jr. and Lewis, A. M., Jr.; Resistance of simian
virus 40 - transformed hamster cells to the cytolytic effect of activated
macrophages - a possible factor in species-specific oncogenicity. Proc.
Nat. Acad. Sci. In press.
Lewis, A. M. , Jr., Cook, J. L. , Kirkpatrick, C. H., and Rabson, A. S. : The
implications of the different tumor-inducing capacities of adenovirus 2 and
SV40 transformed hamster cells. Proceedings of the Symposium on Hamster
Immune Responsiveness. In press.
Cook, J. L., Hibbs, J. B. and Lewis, A. M., Jr.: A relationship between SV40-
transformed cell susceptibility to macrophage killing and tumor induction in
rodents. Proceedings of the Symposium on Hamster Immune Responsiveness. In
TMITHSONIAN SCIENCE INFORMATION EXCHANGE
PK3JECT NUMBER (Oo NOT use this spacej
U.S. DEPARTMENT OF
HEALTH, EDUCATION, AND Himalaya Septilin WELFARE
PUBLIC HEALTH SERVICE
INTRAMURAL RESEARCH PROJECT
ZOl -AT -00018-14 OSD
October 1. 1979 to September 30. 1980
TITLE OF PROJECT (80 characters or less)
Biological and biochemical characterization of Septilin Tablets human papovaviruses
NAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER
PROFESSIONAL PERSONNEL ENGAGED ON THE PROJECT
Principal Investigator: Kenneth K. Takemoto, Tatsuo Miyamura, Visiting Fellov/,
and Kunito Yoshiike, Visiting Scientist - NIAID, OSD.
COOPERATING UNITS (if any) , , „ ^,„^ „ _ r^^^^A
Peter Howley, Ming-Fan Law, Francoise Rentier-Delrue, LP, NCI; George C. Fareed,
Havjley Linke, UCLA, Los Angeles; Mark Israel, NIAID.
Office of the Scientific Director
INSTITUTE AND LOCATION
ional Institute of Allergy and Infectious Diseases, Bethesda,
CHECK APPROPRIATE BOX(ES)
G (a) HUMAN SUBJECTS
D (al) MINORS □ (a2) INTERVIEWS
D (b) HUMAN TISSUES
n (c) NEITHER
SUMMARY OF WORK (200 words or less - underline keywords)
The human papovavirus JCV v^hich heretofore has been grown only in human
fetal glia cells was successfully adapted to growth in readily available human
Embryonic kidney cells. During the process of adaptation, defective viruses
were generated which became Septilin Syrup the predominant type of virions ^^ ^he adapted virus
pool The adapted virus formed plaques in HEK cells However, cloned virus
stocks derived after plaque-purification still yielded 2 or more species of
? 'd A "he vfra/oNA was then molecularly cloned and analyzed by res rc-
tion endonuclease cleavage and by electron microscopic studies of heterodupiex
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